UC Davis Summer Short Course in Flow Cytometry
Sponsored by the UC Davis Biotechnology Program
July 17-21, 2017

This comprehensive flow cytometry course is enhanced with an exciting lineup of interactive laboratory sessions using many of our UC Davis cytometers as well as new instrumentation and software to teach you to a variety of established and new approaches in cytometry. Special guest speakers will introduce new trends in flow cytometry and explain the physical and electronic concepts in light collection and signal processing.

Course objectives:

  • Learn the fundamental concepts of how flow cytometers work
  • Discuss best practices in sample preparation for flow cytometry
  • Describe standard assays and techniques
  • Introduce new cutting edge technologies and applications for single cell analysis and genotyping
  • Demystify multicolor cell staining, compensation and analysis

 
Laboratory sessions include:

  • UC Davis MoFlo Cell Sorter: cell sorting theory and practical considerations 
  • UC Davis LSRII: Software setup for successful multicolor acquisition and analysis
  • Multispectral imaging flow cytometry using the Amnis FlowSight Imaging Cytometer
  • Cell proliferation
  • Multiparameter analysis of cell surface markers on the BD LSRII system
  • FlowJo basic and advanced flow cytometry data analysis and panel design wizard
  • Targeted gene expression in single cells using flow sorting and the Fluidigm BioMark

For more information, please go to http://biotech.ucdavis.edu/ and click on
“Courses”

or contact
Jacki Balderama
jbalderama@ucdavis.edu